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In brief, surgical specimens from the neocortex and hippocampus were fixed in formalin and sectioned perpendicularly to the cortical surface into several tissue blocks.
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The cortical changes identified histopathologically ranged from one to several gyri in one to six tissue blocks (equivalent to between ≤5 mm and approximately 30 mm).
Secondly, several groups (Rome, Boston/Göttingen, Amsterdam) have used in part tissue blocks from the same patients derived from a single tissue bank (UK Multiple Sclerosis Tissue Bank).
To this end, we applied rigorous multiple sectioning of the lymph node tissue blocks to achieve deeper levels, investigated several levels of each lymph node, and increased the sensitivity of tumor cell identification by K19 immunolabeling.
Since July 2003, CPCTR has offered over 6,000 cases (38,000 blocks) of highly characterized prostate cancer biospecimens, including several tissue microarrays (TMA).
Recently, several workers have used germinal centre and activated B cell immunohistochemical markers on paraffin embedded tissue blocks to classify DLBCL into three prognostic groups[ 7, 8].
For example, in a DGH there may be only one histopathologist dealing with several cancer sites and who does not have any protected time to deal with 'trial' tissue blocks in the tight timelines that some trials have.
Several tissue banks are involved.
Histology slides, tissue blocks and autopsy reports were obtained.
In cirrhotic livers, scar tissue blocks blood flow.
Frozen tissue blocks were maintained at −80°C.
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