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Ex vivo assays of an exogenously administered compound in tissues by liquid chromatography and tandem mass spectrometry (LC-MS/MS) have been used to evaluate kinetics of compounds in the brain without radiolabeling and to predict the capability of compounds as PET radioligands for several target molecules, such as opioid κ receptor and nociception/orphanin FQ receptor [16 20].
Ran GTPase activates several target molecules to induce microtubule formation around the chromosomes and centrosomes.
This leads to stabilization and activation of β-catenin, inducing several target molecules such as Myc.
The intercellular messenger nitric oxide has several target molecules in a typical cell.
Each TAS is cleaved by one or two miRNAs, producing several ta-siRNAs in phase and resulting in an amplified signal that silences several target molecules [3,4].
A vast number of molecular studies have identified several target molecules involved in inflammatory changes, and most anti-inflammatory drugs currently used suppress the biosynthesis of the inflammatory mediators mentioned earlier [ 8].
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The conformational flexibility of disordered regions allows them to interact efficiently with several different target molecules [32].
After determination of several key target molecules, corresponding compounds for each molecule could be analyzed.
Recently, with the progress of pathogenic mechanism of ALS, several potential target molecules for therapy have been demonstrated.
Moreover, bioinformatics studies allowed us to predict several candidate target molecules for the future translational studies of this or other phytocompound mixtures.
Indeed, a single activated upstream protein has the potential to activate several downstream target molecules (e.g., ref. [ 38]); therefore, higher levels of selective constraint might be expected at these genes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com