This is the first simulation study to address some of the technology-specific characteristics found in several NG sequencing technologies.
The past 10 years have witnessed a dramatic reduction of DNA sequencing costs enabled by the emergence of several disruptive sequencing technologies.
Deposition of linear DNA molecules is a critical step in many single-molecule genomic approaches including DNA mapping, fiber-FISH, and several emerging sequencing technologies.
In response to the referee comments, the discussion has been edited to acknowledge that several novel sequencing technologies have become available that are likely to or have already superseded microarray resequencing for clinical sequencing.
Several post-Sanger sequencing technologies (also referred to as next-generation sequencing technologies) have since been developed that enable extremely rapid whole-genome sequencing and a broader application of comparative genomics [ 33, 34].
Standard methods and tools for detecting and dealing with common contamination like vector sequences or genomic contamination exist, some of them applicable to one or several second-generation sequencing technologies (Chou and Holmes, 2001; Falgueras et al., 2010; Kong, 2011; White et al., 2008).
Owing to the paucity of genomic data available by the advent of third generation sequencing technologies, several 'omics' technologies were applied to understand the underlying evolutionary pattern and lifestyle of the bacterial pathogen using phylogenomics.
Recently, several 'next-generation' DNA sequencing technologies have become commercially available [ 33- 35].
Triggered by advancements in sequencing technologies, several crop genome sequences have been produced or are underway [ 79 82].
