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While we detected several sequence alignments to spirurid nematodes in both the DNA and RNA sequence data, none of these species are known to be present in the US or to infect taurine cattle.
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Target transcripts of these probes were annotated using several sequence alignment algorithms.
With the new datasets of BRAliBase 2.1 we tested several sequence alignment programs.
If activated, ReXSpecies 2.0 can employ several sequence alignment tools to redo the alignment, per default muscle [ 61] is used.
Sparse indexing is not unique to suffix arrays, and in fact is used by several sequence alignment tools, e.g. BLAT [ 33], indexed MegaBLAST [ 34].
3. On page 13 in Methods the authors list several sequence alignment programs that are tried and also admit to "some manual editing in Bioedit".
These examples are intended to be illustrative rather than exhaustive, since there are several sequence alignment editors to choose from, many of which are optimized for specific tasks other than those addressed here.
We also investigated the patterns of FOXI1 protein evolution from several multiple sequence alignments, including additional non-human primates besides chimpanzee.
Next, we tried out several different sequence alignment and structural superposition software packages to align the entire proteins.
Candidate myotubularin sequences (including both the PH-GRAM domain and the myotubularin phosphatase domain, or just the phosphatase domain alone (as defined by the sequence of the solved structure of MTMR2_Hu (PDB: 1LW3)) were aligned utilizing as necessary several multiple sequence alignment programs: Muscle [ 58], T-Coffee [ 59] or M-Coffee [ 60, 61].
For example, UniProt (Swiss-Prot and TrEMBL) and the Gene Expression Omnibus (GEO) all directly link into BLAST; GenBank links into several multiple sequence alignment tools, while the Protein Data Bank (PDB) links into various protein prediction and evaluation programs.
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