Sentence examples for several reference gene from inspiring English sources

ORA techniques tend to have low discriminative power (for a target gene set, several reference gene sets receive the same or similar significance scores, e.g. see Table 1) and the scores vary considerably with small changes in the overlap size.

Generally, normalizing expression of the target genes to one or several reference genes provide an efficient way to reduce these effects and increase the relevance of the results (Huggett et al. 2005; Marabita et al. 2016).

For the analysis of the global NBPF expression pattern, we determined relative gene expression levels using an optimized two-step SYBR Green I RT-PCR assay employing several reference genes [19].

Several reference genes (18S, GAPDH, ACTB and PPIA) were tested for mRNA quantification.

We identified several reference genes that are suitable for qRT-PCR data normalization in Brachypodium.

Several reference genes (18S, PLC4, GAPDH, ACTB and PPIA) were tested for mRNA quantification.

This relative quantification was reliant on the use of several reference genes: 18S RNA, G6pdx, Gapdh, Tcea, Tbp.

For large RNAs, the stability of several reference genes has been widely reviewed [ 5– 8] and even called into question [ 9– 11].

Good practice in data normalization for gene expression analysis therefore relies on the identification, experimental validation and use of several reference genes.

A selection of several reference genes used simultaneously can also be a good way to further increase reliability of the resulting data [ 50, 51].

Several reference genes are commonly used, such as elongation factor [ 24, 25], actin [ 26, 27], ubiquitin [ 28, 29], and ribosomal units (18S or 28S rRNA) [ 30- 32].