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Second, the exploitation of an additional level of parallelism in order to simultaneously align several query sequences with the same reference sequence, targeting the processing of short-read DNA sequences.
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We performed several BLAST searches with various GFP query sequences but did not find evidence for any GFP homologs in the Mnemiopsis genome.
TBLASTN searching of the T. brucei GeneDB with these query sequences revealed several predicted genes with only short stretches of alignment.
More complete list is available in Additional file 2. For the vernalization pathway, we were not able to find FLC-like sequences from our EST libraries or public Fragaria or Rosaceae EST databases by tBLASTn searches although we used the FLC and FLC-like sequences from Arabidopsis (MAF1- MADS, MAFFECTINGTING FLOWERING 1-5) and several other plant species as query sequences [ 13, 50, 51].
For the PSI-BLAST scans, Pfam [46] domains IBN_N (PF03810, Importin-β N-terminal) and Xpo1 (PF08389, Exportin 1-like protein), which are specific to several KAP-β family members, were used as query sequences in blastpgp (PSI-BLAST) [45]) searches.
Other searches used as query sequences the PRP8 protein sequences from several fungi.
The search algorithm accepts several query types (e.g., PDB code, PDB header, linker length, secondary structure, sequence or solvent accessibility).
What other query sequences?
from initial exploratory query sequences.
ABBA PTases used as query sequences.
Several query combinations were used using the MeSH terms.
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