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It encodes several protein isoforms through alternative splicing of two exons: 8b and 9b.
CAR exists in several protein isoforms including two transmembrane isoforms that differ only at the carboxy-terminus (CAREx7 and CAREx8).
Although this is likely, such a correlation may be complicated by the fact that most TRIM genes, if not all, encode several protein isoforms, which may have different sub-cellular localization and stability.
However, the p63 gene encodes several protein isoforms generated by alternative splicing.
TMPO (lamina-associated polypeptide 2 gene) codes for several protein isoforms, the most studied of which are LAP2β and LAP2α, with marked differences in their cellular roles (Berger et al., 1996; Dechat et al., 2000).
Similar to the exocarp data, we identified > 2-fold accumulation of several protein isoforms annotated with functions in cell enlargement (e.g. expansins, aquaporins), fruit softening (e.g. polygalacturonase, pectate lyase) and defense (e.g. pathogenesis-related proteins) concomitant with a significant reduction in proteins inhibitory to fruit ripening (e.g. polygalacturonase-inhibiting protein).
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To detect the potential presence of various protein isoforms we prepared lysates of several cell types and did Western blot with an antibody specific for the carboxy terminus, which is common to all three isoforms.
Heterogeneous mitochondrial network alterations in glioma cells and in microvascular environment are implicated directly and indirectly in the processes linked to hypoxia-tolerant and hypoxia-sensitive cells phenotype, effects of the hypoxia-inducible factor-1α, increased expression of several glycolytic protein isoforms as well as fatty acid synthase, and survivin.
Similarly to p63, p73 is expressed as several distinct protein isoforms.
C/ EBPβ is expressed as several distinct protein isoforms (LAP1, LAP2, and LIP).
This study led to the global characterisation of S100 protein expression in thyroid tumours and highlighted several S100 protein isoforms for potential diagnostic and therapeutic evaluation.
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