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There are several probe design strategies for microbial community profiling; we prefer designing probes to detect the ribosomal RNA (rRNA) with specificities to detect the common regions of sequence shared at each phylogenetic rank, and thus approximate taxonomic rank (e.g. species, genus, phylum, and domain).
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Sequences that appear to be present in several probe designs of microarray platforms (table 1) indicate that no design rules exist today to preclude them.
Analyses of the 59K unigene set allowed for several different probe design possibilities.
The method was tested with several suction probe designs and withdrawal pressures using coalescing and non-coalescing model media as well as during yeast fermentations.
Arrays for detecting alternative splicing are available using several different probe designs, including those based on exon-junctions.
However, if several probes were designed from a FL-cDNA locus or a CDS, the highest signal intensity among the probes was defined as the signal intensity for the locus or CDS.
Our results highlighted several key features of probe design: a shorter linker was more optimal for both Eu III) ion emission intensity and cellular uptake; the folic acid targeting motif exhibited higher cellular uptake when compared to pteroic acid; the emission intensity of the folic acid based probes was pH insensitive, whereas the pteroic acid based probes were pH sensitive.
Several tools are available for probe design.
The MTRIP probe design has several distinct advantages over other virus-labeling methods.
Therefore, we decided mining transcript information for probe design from several databases.
We also determined that several different measures commonly utilized in probe design were not predictive of outlier probes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com