Present study followed an in-silico based methodology, involving scanning and filtering the complete proteome of Rickettsia rickettsii by using several prioritization parameters in the search of potential candidates for drug development.
After the gene retrieval, several prioritization criteria were used to identify potential causative genes.
Several prioritization steps were taken to decrease the number of genetic variants and to find the potentially pathogenic variants [ 10].
Several prioritization methods assess the probability of a gene or protein to be generally associated with some disease, but are unspecific for the disease.
Several prioritization methods have been proposed for and applied to human pharmaceuticals (e.g., Kostich et al. 2010; Kostich and Lazorchak 2007; Sanderson et al. 2004) and veterinary medicines (Boxall et al. 2003b; Capelton et al. 2006; Kools et al. 2008).
Evolutionary and phylogenetic distinctiveness are fundamentally important aspects of biodiversity (Diniz-Filho et al. 2013), and several prioritization schemes rank conservation priority based on distinctiveness (Taylor et al. 2011; Rosauer and Mooers 2013; Jetz et al. 2014; Vokmann et al. 2014).
