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To understand how ligand binding relates to structure, the secondary structure of Plin2 was predicted using several prediction programs including PredictProtein, PSIPRED, SAM, and SABLE2.
OsFKBP46 and OsFKBP57 that have no signal motifs were predicted to be cytosolic, whereas OsFKBP73, with its long Arg/Lys amino-acid-rich domain, was predicted to be nuclear by several prediction programs.
Subcellular localization of deduced LOX proteins was predicted through the identification of signal peptide sequences using several prediction programs such as ProtComp (http://www.softberry.com/berry.phtml), PSORT (psort.nibb.ac.jp/).nibb.ac.jp/
Approximately 25% of the mRNAs downregulated by ING1b are also predicted to be target genes of miR-203 using several prediction programs.
All four novel mutations were examined by several prediction programs [ 17].
This is based on the fact that we have used several prediction programs in order to search if these regions could correspond to "consensus" sequences previously known.
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Approximately 8% of annotated repeats represent collapsed regions with coverage several times higher than average, so the real repeat content may be higher.> -wrap-foot> Ab initio gene predictions were conducted with several gene prediction programs, resulting in 10,907 predicted gene models.
Promoters were predicted for G72 and G30 by collating and analysing results from several promoter prediction programs.
The conserved sequences were then submitted to several epitope prediction programs, whose results are combined.
To test if this is possible with the currently available promoter predictors, we evaluated several promoter prediction programs in their ability to accurately determine NTLs (i.e. how well they perform at or very near 100% sensitivity) and compared their performance to the NTL determination performance of DDM.
A better estimate of the true topology can be achieved through the use of several different prediction programs.
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