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Digitoxin appeared most potent and IC50 values for several patient samples were at concentrations that may be achieved in the clinic.
However, only the outlying degree can identify genes dysregulated across several patient samples.
62 63 This short questionnaire proves to be a valid and reliable measure to determine the impact of fatigue in several patient samples (eg, multiple sclerosis, cancer, Parkinson's disease, post-stroke and chronic fatigue syndrome) 81 85 and to detect change over time.
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8 Several negative patient samples (unrelated to the present study) were included in each batch to rule out any contamination.
The influence of several patient and sample characteristics on the sensitivity of the selected assays was analyzed by univariate analysis (Technical Appendix Table 2).
Several previously extracted patient samples were retested by the IS481 HP assay to ensure the integrity of the extracted DNA over time (data not shown).
Quantitative mass spectrometry targeting specific molecules rather than random sampling of the whole proteome, e.g., multiple reaction monitoring (MRM), offers an efficient and effective means to multiplex the measurement of several candidates in patient samples, thereby omitting the need for antibodies prior to clinical assay design.
A total of 84 gene targets (and the 18S rRNA housekeeping control gene) were chosen for confirmation of expression in patient samples, several assessed with redundant probes.
In addition to BrCa patient samples, several EV proteins have been identified from immortalized cancer cell lines.
To further assess if these in vitro drug treatments mimicked in vivo behavior, we undertook the expression analysis of ABC transporters in several chemotherapy-treated primary patient samples.
Here, we used patient samples and several CRC cell models to reveal a unique role for IGFBP-rP1 in inhibiting EMT and metastasis via a TGF- β/smad-dependent pathway.
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