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Exact(8)
Several nucleotide changes, including a 16-bp deletion in the 5′-UTR, a 6-bp deletion in the first exon, and three nucleotide changes resulting in amino acid variation in the first and fourth exons of the GKBR allele, were revealed (Fig. 1).
crtS gene sequences from strains VKPM Y2410, ATCC 24203, ATCC 96594, and ATCC 96815 show several nucleotide changes, but none of them causes any amino acid substitution, except a G2268 insertion in the 13th exon of ATCC 96815 which causes a change in the reading frame.
However, in the region encoding the RelE2Spn toxin, several nucleotide changes were detected.
Correct placement of ArEq003 is not clear because of several nucleotide changes.
However, a single nucleotide change within the proximal promoter and several nucleotide changes within the 3'-UTR were identified.
The sequence obtained for pUC-STS was found to match the published sequences of sts from peanut[ 21, 22], but with several nucleotide changes.
Similar(52)
Each of the mt pseudogenes is a partial sequence of a protein-coding gene or an rRNA gene with several nucleotides changed, and has a high similarity (>85%) to the homologous sequence in functional gene (Table 1).
A second copy of the gene, SMN2, is almost identical to SMN1 except for several single nucleotide changes, one of which results in alternative splicing and skipping of exon 7 [8], [8] so that 75 90% of SMN transcripts from SMN2 are truncated and are rapidly degraded in vivo [9].
DQ289479– DQ289486) showed insertions of 36 and 4 nucleotides exclusively in the lung, several point nucleotide changes specific to lung/heart or testis/ovary besides a few randomly distributed ones across the tissues (Additional file 5).
Sequencing of mutation sites showed that even though several single nucleotide changes were found in QM9123 and QM9414, other types of mutations (e.g. deletion of few nucleotides) were more frequent than in NG14 and Rut-C30.
Among them was a novel 800 bp transcript (GenBank accession no. EF051520 and EF050082) harboring an insertion of 18 bp at one place exclusively in the spleen, and several point nucleotide changes in sperm/kidney (Additional file 10).
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