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Several negative controls in nested PCR amplification experiments yielded amplicons, which were then cloned to contribute establishing a catalogue of laboratory and/or PCR reagent contaminants.
The Applied Biosystems 7500 fast real-time PCR system was used, all samples were run in duplicates and each run contained several negative controls (no template) and a reference sample.
Several negative controls were made to support the specificity of the immunogold procedure.
In addition, during the procedures of DNA/RNA isolation and amplification, several negative controls are included to monitor for possible false-positive findings.
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Several negative control spots, including a random 70 mer pool and randomized hexamers, were included to assess non-specific hybridization.
The competition assay was first tested on a pilot array, comprised of MBD1, MBD2, MeCP2, and several negative control proteins.
One positive sample and several negative control samples were included for each infectious agent, which were treated identically to the virus samples throughout.
Data quality was assessed using several assay controls and Detection p-values were computed using several hundred negative controls to determine gene expression detection limits.
Detection p-values were computed using several hundred negative controls to determine gene expression detection limits.
Multiplex PCR amplifications were performed on the Coriell genomic DNA samples (plus several negative PCR control samples that contained no genomic DNA).
Each microarray contained several control spots positive control spots (containing olignucleotides from several P. yoelii genes), mouc+ spots (containing oligonucleotides from mouse genes), several different negative control spots (containing oligonuclotide with random sequences), empty spots (containing no oligonucleotides) and 3xSSC spots (containing 3xSSC).
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