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One out of billions of cells acquires a mutation, or several mutations
Several mutations which are reported to be lethal in animals have been found to be non-lethal in Arabidopsis.
Screening of TOLLIP gene by sequencing and SSCP analysis [ 26, 30, 40] has shown several mutations, which affect gene expression and biological function modifying inflammatory-cytokine production.
In our study however, several mutations, which may cause moderate to high level of resistance to the NNRTIs and NRTIs were detected, indicating a changing pattern and a rise in transmitted resistance to antiretroviral drugs in Ethiopia.
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The phenotype was specifically caused by Abp1 deficiency because cotransfecting the cells with an Abp1 construct carrying several silent mutations, which render the mRNA resistant to RNAi, restored the wild-type situation (Figure 1D).
In normal, healthy controls and AD patients, we identified several missense mutations, which may have affected the activity of SASPase.
Therefore, additional marker strains were constructed containing several auxotrophic mutations, which allowed the determination of genetic distances between these markers.
Overall, in addition to the Ile → Val mutants, we also utilized several 'nudge' mutations, which helped to resolve assignment ambiguities.
Several other mutations which are typically associated with or acting in parallel with SA also partially reduced cell death in dnd1 and included eds1, pad4, ald1 and fmo1 (Table 1; Additional file 2).
This work could raise a common mechanism for several FTDP-17 mutations, which could act through the dysregulation of Tau phosphorylation status ultimately leading to conformational changes.
Transient neoplastic tumor cells frequently have mass chromosomal alterations, rather than several dozen genetic mutations which eventually lead to drug resistance [ 13].
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