We selected several mutants, each with a single gene knockout.
Several mutants also showed large and complex deletions in multiple locations within the LacI target.
For thermotolerance, several mutants were more heat tolerant than their wild-type parents.
We obtained several mutants with higher activity, del1 2, del1 3, and S2R.
It has been reported that leaf photosynthesis is affected in several mutants with a rolled leaf phenotype.
Biotechnological production of the serinol derivatives sphingosine, dihydrosphingosine or phytosphingosine has already been established with several mutants of Pichia ciferri.
Several mutants (AcN-Q339K, AcN-Q343K, AcN-T201F, AcN-T201W, AcN-T201L, AcN-T201I) were obtained with dramatically improved thermostability.
It is known that wild-type and several mutants of CYP102A1 could oxidize several human P450 substrates, including pharmaceuticals (Yun et al. 2007).
From the several mutants studied, a mutant "Mut-4" was selected and studied based on the higher bikaverin production and high specific productivity.
Recently by mutation breeding, several mutants with low phytate content have been developed and are good resources as low-phytate donors in breeding programs (Liu et al. 2007).
To investigate the structural determinants for coenzyme specificity, several mutants involving residues Gly206, Arg207 and Arg208 were engineered and kinetically characterized.
