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Our analysis confirms that methapyrilene-mediated hepatotoxicity is accompanied by induction of genes involved in protein translation, structural remodeling, cell death/apoptosis, and the repression of several metabolic processes including lipid metabolism, steroid metabolism, and fatty acid β-oxidation.
In addition, SA treatment promoted the induction of a subset of genes involved in several metabolic processes, such as redox states and secondary metabolism, which are associated with biotic stress.
According to functional annotation, genes of cluster A, which exhibited a common pattern of increase in expression from E13 to E15, were involved in several metabolic processes and pathways, most of them associated with lipid metabolism and protein transport.
Several metabolic processes are connected in this network, e.g. carbohydrate and lipid metabolism with Pcsk5 catalycing reactions ranging from precursors for growth factors, such as Figf, to cell surface receptors and viral surface glycoproteins [186].
Cr III) at increased concentrations can interfere with several metabolic processes because of its high capability to coordinate various organic compounds resulting in inhibition of some metalloenzyme systems (Zayed et al. 1998).
However, because of their simple growth needs, they are the favourite model organisms for deeper understanding of several metabolic processes and for the production of recombinant compounds of medicinal and commercial value.
Other processes that were overrepresented in this subset are muscle contraction and several metabolic processes.
Topoisomerase II (Top2) is a nuclear enzyme involved in several metabolic processes of DNA.
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Several metabolic process related gene categories such as 'phenylpropanoid_metabolic_process', 'secondary_metabolic_process' and 'flavonoid_biosynthetic_process' were up-regulated in C0 versus C1 comparison (Additional file 4) and the same gene categories were down-regulated in C1 versus S1 comparison (Table 4).
An enrichment analysis using Agrigo [ 26] of the 206 genes that had GO annotations in the A. thaliana TAIR10 database revealed that seven biological functions were significantly over- represented (FDR < 0.001; Additional file 9), specifically catabolic process, cellular component organization, developmental process, multicellular organismal process and several metabolic process categories.
Up-regulation of several metabolic process genes between the control samples and down regulation of these gens under stress treatment may reflect the reduction in growth under stress conditions suggesting that these genes play a role in normal plant growth and development.
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