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This enhanced cultivation system produced rapid growth of several isolates of campylobacteria from water and chicken samples.
The movement protein genes from several isolates of ArMV and GFLV of different geographical origins were amplified by RT/PCR using degenerate primers, cloned and sequenced.
Several isolates of Trichoderma spp. have been characterized and evaluated against different fungal pathogens of pulse crops (Dubey et al., 2006; 2007; 2009; 2011; 2012; 2013; Jamali et al., 2004 Chaudhary et al., 2004; Mishra et al., 2015).
Spot tests on skim milk agar revealed several isolates of outstanding proteolytic activity, exhibiting clear zones width around colonies within a range of 5.5 and 10.5 mm (Additional file 1: Figure S1).
Several isolates of C. coccodes, 15 species of Colletotrichum, a variety of heterogeneous organisms and various plant species were first screened by RAPD-PCR, and a strain specific marker was identified for C. coccodes (183088).
Specificity of the primers was tested against several isolates of various anastomosis groups (AGs) of BNR, other related and unrelated fungal species, and roots not infected or infected with fungi.
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Whole genome sequencing has been completed for several isolates to investigate the origin of the Haiti cholera outbreak (4, 5 ).
For the several isolates, existence of plasmid was confirmed to isolate plasmid DNA using a Miniprep DNA purification kit (Takara).
Infectious phage were found in culture supernatants and increased ≈1,000-fold ≈1,000-foldcin C. withe-genomitomycinC.ng of several isolates and PCR analyses of all strains confirmed that stx 1a was carried by a lambdoid bacteriophage.
However, several isolates (including some of G1407 and G225) were non-susceptible to AZT (MICs of 0.38-0.75 mg/L).
This could suggest the presence of several isolates within Norrland, and indeed, signs of such were detected in the MDS and FST analyses.
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