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Mann-Whitney's (two independent samples) and Kruskall Wallis (several independent samples) tests were used for comparisons.
Data are represented as mean ± SEM from several independent samples or experiments.
However, with our approach we did find consistent association to genetic variants in several independent samples for MC5R, DYM and NEDD4L.
Identifying "clustered" positive findings at several nearby SNPs and finding clustered positive results in several independent samples provide some of the best available controls for technical errors and for the large numbers of repeated comparisons that are fundamental to GWA.
The reactions were run in triplicate and repeated on several independent samples for each genotype.
Different alleles of two SNPs in the Engrailed 2 (EN2 -gene on chromosomEN2 -genere assoniated with AD in several independent samples [ 11, 29, 84, 90].
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Population studies have highlighted SNPs in some of them in association with growth related phenotypes., - Among several independent placenta samples, a perfectly monoallelic expression could be seen for five of the seven candidate genes, showing a strict imprinting.
First, mtDNA mutations that became recurrently fixed in several independent cancer samples recapitulated ancient mtDNA genetic backgrounds (haplogroups), thus suggesting commonalities in the selective constraints acting on the mitochondrial genome in cancer and during human evolution (Zhidkov et al. 2009).
When there exist several independent and heterogeneous samples, different in case selection, geographical location, race, genotyping, etc., or even sample size, applying a multiple replication group strategy may help eliminate false positive signals and select results consistent across groups.
We recognize a preferable approach would be to sequence several distinct and independent samples from each symbiont state.
Several independent studies of samples from diverse geographic origins have shown that the healthy honeybee gut contains a specialized microbial community, dominated by eight distinct phylotypes [ 4, 12- 14].
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