There is an unusual number of non-coding regions and their function, if any, is not known; however, several of these demark abrupt shifts in nucleotide skew, and there are several identical sequence elements at these junctions, suggesting that they may play roles in transcription and/or replication.
Taxa with several almost identical sequences, only the sequence displaying the shortest branch length on the tree was used in subsequent analyses.
In fact, 20,831 out of 22,162 (94 %) base pairs in the low coverage regions correspond to the coding region of rRNA, tRNA, and ribosomal proteins, which have several nearly identical sequences on the E. coli genome.
Additionally, several groups of identical sequences are detected that indicate sharing of various MIB loci by different Mamu-B serotypes (Fig. 4, Table 1).
The close genetic relatedness has been confirmed with sequencing of several housekeeping genes showing identical sequences for Norwegian isolates [ 18].
It should be noted that GmSEOu and GmSEO-F2 share several identical forisome-specific peptide sequences, so GmSEOu is likely to be involved in the formation of forisomes.
Indeed, our data contain several examples of species having identical sequences in V9 region (for example in the genus Phaeocystis).
Several groups of clones shared identical sequences (groups of nine, seven, five, five, two, two, and two clones for the VT-like genotype; and groups of two and two clones for the T30-like genotype) while all T36-like clones had unique sequences.
This has in some cases resulted in the inclusion of seemingly identical sequences in several of our trees as in those cases distinct and unique sequences were represented only by their conserved domain regions.
