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This is because the studied accessions were inbred by selfing for several generations prior to sequencing (table 1).
Furthermore, because he did not interbreed the mixed origin brood stocks for several generations prior to the start of the exploitation trials, effects of genetic linkage disequilibrium may have influenced the results.
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These gltA1 allele dynamics suggest that the clade that gave rise to Cit+ was rare within the population for several thousand generations prior to the evolution and refinement of this metabolic innovation that enabled it to achieve numerical dominance.
A factor that may have contributed to the lack of an effect in these two studies is the high level of inbreeding measured, with several generations of full sibling inbreeding occurring prior to assays of sex ratio.
Prior to testing, all mutations were out-crossed for several generations to a strain bearing an X chromosome that carried a null mutation of the w gene and then crossed to our 39C-5 bearing strain.
Based on the review of prior studies, we arrived at the theoretical assumption that the coresidential living arrangement of several generations is no longer the binding cultural ideal in today's China.
Prior to the experiment, each aphid line was reared on multiple plants, chosen haphazardly, from a cohort of healthy plants of similar age and size for several generations in 16 L:8D intervals at 20°C in a Percival I-41LLVL environmental incubator to reduce effects resulting from variation in prior culturing (i.e. maternal and grandmaternal effects).
There are several generations each season.
There may be several generations per year.
Several generations of greenstones have been recognized.
Gone, even, are several generations of renovations.
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