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However, in this method, several flasks need to be prepared under similar conditions.
The foam was channeled through several flasks with distilled water to collect the produced biosurfactant.
The culture was split into several flasks and incubated at 21°C.
Articular chondrocytes from rats were seeded into several flasks, and the media in one-half was supplemented with glucosamine at 20 mM.
Chondrocytes isolated from the cartilage of rats were cultured in several flasks either alone or in the presence of 20 mM glucosamine.
(If necessary, the cells from several flasks can be pooled at this stage). The cells should now be counted and the final count adjusted to 2 × 105 per millilitre by the addition of more growth medium.
Similar(52)
Currently, to monitor the environmental variables, several sample flasks are prepared and cultured simultaneously, and a single flask is retrieved from the shaking table platform periodically and sampled aseptically to measure the time course of the shake-flask culture of microbes.
The developmental stage of several representative flasks of worms was confirmed microscopically before commencing the experiment.
For large-scale production, several 2-L flasks of Tn5 cells were grown to a density of 2.0×106 cells per mL, which were then infected with recombinant virus at a multiplicity of infection (MOI) of 10.0.
For measuring DOT in shake flasks, several techniques have been introduced so far.
Abdullah sometimes carried in our twice-daily meals — a couple of tins of tuna, several buns, a flask of sweet tea and a mango or a few soft bananas.
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