Milligram quantities of GPCRs can now be expressed in several expression systems and purified to high homogeneity.
However, a synthetic version of MVP (MVP-s), in which 62 of the archaeal codons were altered to bacterial codons (see Experimental Procedures), was successfully expressed in several expression vectors and cell lines (Table 1).
Recombinant cathepsin L could not be expressed despite the use of several expression vectors and E. coli strains (results not shown).
To test if ELF6 and AtJmj4 are active histone demethylases, we tried to express the full-length ELF6 and AtJmj4 proteins in several expression systems.
However, attempts to produce functional recombinant MAPs (mainly foot protein type 1, fp-1) by several expression systems have failed.
By using the PCR strategy, it is possible to create several expression cassettes and simultaneously screen for the best target sites on any given mRNA.
Although several expression architectures based on foreign RNA polymerases have been established for this organism, all of them rely on integration of the expression cassette into the genome.
Despite the apparent uniformity in cellular composition of the mammalian cerebellar cortex, a complex topography is revealed by several expression patterns.
Several expression plasmids with improved efficiency for the transcription of the pac gene and/or translation of the pac mRNA were constructed.
By exploring several expression systems and designing a number of constructs, we were able to purify the enzyme in its active form and we found that it is tightly bound to the membrane through the N-terminal residues.
Several expression vectors for different forms of surface display have been developed and, coupled with improved recombination strategies, represent progress towards a refined tool for use in functional genomics and in vitro protein evolution.
