Exact(2)
We found several contigs that each contain highly homologous members of the same CR families, which is consistent with findings in gnathostome genomes where CR genes often cluster in specific chromosomal regions [ 8].
We identified several contigs that match cardinal sex determination and differentiation genes (e.g. dsx, fru), or have been implicated in JH or ecdysteroid function (e.g. epoxide hydrolase 1, EcR, ftz-f1, usp).
Similar(58)
This resulted in several assembled contigs that were just under 200 bp (but most were between 400 and 600 bp) to be included in the alignment.
On the other hand, there are several juvenile contigs that are absent from the adult database, although represented in other organisms suggesting that they might represent stage specific transcripts.
For the pig autosomes many BACs consist of several, often unordered, contigs that are not finished to a high quality.
This in silico analysis was validated by performing qPCR analyses on several of the contigs that showed differential gene expression levels based upon the pair-wise in silico analyses.
After a detailed analysis of sequence alignments, several contigs and/or singletons that were not automatically assembled were considered to encode a same putative protein (Table 3).
The frq1 genes of D. sechellia and D. pseudoobscura are situated in genomic contigs that contain several gaps filled with 'N' and they are not complete.
Although viral metagenomics approaches have both expanded our view of global viral biodiversity, and paved the way towards a better understanding of viral ecology and evolution, several methodological biases undermine the effective analysis and taxonomic assignment of the sequence reads and contigs that have been generated using these approaches11,19,20.
Several groups used CD-HIT to remove redundant contigs from de novo contig assemblies by removing contigs that showed homology; CD-HIT is a program that selects as a representative sequence the longest contig in each cluster of contigs [ 21, 23– 25].
The leftover contigs were checked by BlastN versus the template, and two contigs that were not covered by any part of the template were extended by several rounds of assembly versus the 454 reads until they were closed.
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