Altogether, our results showed that adaptation to climate in P. glauca involves many mechanisms playing at several cellular and organismal levels.
These findings suggest that TH may regulate the cellular levels of several species of miRNA which, in turn, regulate the mRNA levels of some genes.
Nme5, also known as the inhibitor of p53-induced apoptosis-beta (IPIA-beta) is known to confer protection from cell death by Bax and alter the cellular levels of several anti-oxidant enzymes such as Gpx5 [ 34].
Although there is no measurable difference between Rps27l +/+ and Rps27l−/− MEFs (two independent pairs) in total cellular levels of several ribosomal proteins known to bind to Mdm2, including Rpl5, Rpl11, Rpl23, Rps7, Rps14, Rps19, we did detect in Rps27l−/− MEFs an increased Mdm2 binding of Rpl11, Rpl23, Rps7, Rps14, and Rps27, but not Rpl5, Rps27a, and Rps19.
The issue in such context – cell death or survival – seems to depend on the cellular levels of several pro- or anti- apoptotic molecules, many of which belong to a family of bcl-2 homologous proteins (Oltvai et al, 1993; Shimizu et al, 1999).
In this regard, USP9x has been shown to modulate the levels of several cellular proteins [22], [23], [24].
Importantly, we noticed that the depletion of ESCRT components did not affect the levels of several cellular proteins, including HSP70, Chk2, and b-actin (Fig. 1A).
Increasing concentrations of Ponasterone A lead to increased O-GlcNAc levels on several cellular proteins (Fig. 3a).
Meanwhile, the expression levels of several cellular genes known to be regulated by β-catenin/TCF were all reduced after treatment with DOX.
