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Another problem we have encountered concerns panning against ligands for which the bacteria are known to encode several different proteins or proteins with several binding domains.
More precisely, halothane overlaps several binding domains found for mecamylamine enantiomers in the α4β2-TMD, including residues α4-Val236 α4-Val2369, and α4-Leu239 (at the α4-Leu249 α4-Leu249e), β2-Leu233 (at the β2-intersubunit site), β2-Lys260 (at L2), and α4-intrasubunit2′).
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Uhrf1 is an interesting multidomain protein that contains several chromatin binding domains, including a tandem Tudor and PHD domain that together bind H3K9me3/me2/H3K4me0 [83,84], and an SRA domain that binds to hemimethylated DNA [85 88].
Fibrinogen binds to a wide variety of nanomaterials because it has several different binding domains that accommodate different nanomaterials [19].
Human La autoantigen is a dimer and contains several RNA binding domains [24].
Interestingly, CAP350 has been shown to stabilize microtubules via several microtubule binding domains suggesting that it could also stabilize centriolar tubules since CAP350 is associated with centrioles [16].
RBPs possess several RNA binding domains such as the RNA-recognition motif (RRM), serine/arginine-rich splicing factors (SR), K-homology domain (KH), and RGG box domain [48].
Individual reader domains are sensitive to the presence of histone modifications close to their main target residue, and most chromatin associated proteins contain several histone binding domains.
Structurally, several adaptor binding domains overlap on the β-arrestin surface, suggesting that some β-arrestin-based signalling complexes must form to the exclusion of others [2].
This effort involved the construction and screening of 3 distinct libraries: a domain library that included several engineered binding domains isolated by phage-display; a lower-resolution linker library; and a higher-resolution linker library.
hnRNP I is an RNA binding protein that carries several RNA binding domains and is well known for its role in mRNA splicing; it is relatively abundant in the cell.
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