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Several antisense transcripts were also detected at day 55 of embryo development (DT) and appear to be connected to processes involving interactions of ABA with other phytohormones.
The function of intragenic transcripts in bacteria is poorly understood, although several antisense transcripts have been shown previously to regulate expression of the overlapping mRNA [ 28].
Several antisense transcripts have previously been identified, such as Emx2os [ 66] and Wt1AS [ 62] where both antisense has been shown to positively regulate the respective sense transcript expression.
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Finally, several natural antisense transcripts, termed ghrelinOS (ghrelin opposite strand) transcripts, were demonstrated via orientation-specific RT-PCR, 5' RACE and in silico analysis of ESTs and cloned amplicons.
Interestingly, in contrast to the E. coli and H. pylori results and our identification of several examples of antisense transcripts generated by read-through into convergent genes, the A. phagocytophilum whole genome study identified only one gene, p44, associated with this phenomenon [23].
For M. avium (including MAH and MAA), two riboswitches as well as several antisense and intergenic transcripts have been identified (Ignatov et al. 2013).
Recently, several groups have identified clustering of short antisense transcripts immediately upstream of transcription start sites (TSSs) [ 22, 23].
Using the 16 rM threshold, we detected 84 antisense transcripts to several known ORFs (listed in Figure 10 source data 1), including the nucleoside transporter pfNT4 (PFA0160c) depicted in Figure 10A.
This class includes previously known classes of ncRNAs including the large intergenic noncoding RNA, transcribed pseudogenes, antisense transcripts and several others, including the annotated classes of functionally distinct transcripts such as Xist, which is involved in X inactivation (12) and Hotair (13), involved in epigenetic regulation.
Several studies have shown that natural antisense transcripts (NATs) are involved in the regulation of gene expression in eukaryotes [35,36].
After cloning and sequencing, we demonstrate that the size variability is due to several splicing processes to which most antisense transcripts (91% in GIII and 74% in Oregon-R) are subjected.
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