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Essoussi et al. compared several alignment tools, indicating that no single tool can consistently outperform other ones by the estimation of quality alignment [35].
In the figure, for several alignment tools, the relationship between the mapping quality reported by the alignment tool and the actual mapping accuracy (the fraction of accurate mappings among those that are assigned the respective quality score) is shown.
In this paper the author sets out to investigate the performance of several alignment tools and to assess their ability to accurately detect known mutations when used in a variant calling pipeline.
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Several NGS alignment tools are based on this method.
If activated, ReXSpecies 2.0 can employ several sequence alignment tools to redo the alignment, per default muscle [ 61] is used.
Sparse indexing is not unique to suffix arrays, and in fact is used by several sequence alignment tools, e.g. BLAT [ 33], indexed MegaBLAST [ 34].
For example, UniProt (Swiss-Prot and TrEMBL) and the Gene Expression Omnibus (GEO) all directly link into BLAST; GenBank links into several multiple sequence alignment tools, while the Protein Data Bank (PDB) links into various protein prediction and evaluation programs.
To determine sources of sRNAs, an annotation pipeline was built using several alignment and prediction tools.
Several multiple sequence alignment tools are available for evaluating the evolutionary conservation of amino acids across gene families and species.
Reads can then be aligned using existing SOLiD alignment tools.
The performance of L_RNA_scaffolder can be improved in several ways with the further development of sequencing technology and alignment tools.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com