It consists of an optical setup with a light source, AAO sensing platform placed into a microfluidic cell, charge-coupled device [CCD] spectrometer device for the detection of reflected optical signal, and PC for the quantification/presentation of signal by fast Fourier transform [FFT] analysis.
To further substantiate this finding, we tested WT and S2−/− animals in an Aschoff type I setup applying a light pulse 2 hrs after activity onset under DD conditions (Circadian time (CT 14) when phase shift transients are more easily assessable.
Reflectivity spectra of PSi optical structures were obtained by a simple experimental setup: a white light was sent on PSi samples through a Y optical fibre (Avantes, Apeldoorn, The Netherlands).
If you're going to use a form of "punishment training", setup a strobe light near the cage controlled by a remote control switch.
For fluorescence microscopy, wet mounts of stained infected erythrocytes or enriched parasites were observed using an image analysis setup consisting of a light and fluorescence microscope (Olympus BX60, Japan) coupled to a microscope digital camera (Olympus DP71, Tokyo, Japan) and images were captured using computer software DP Manager version 3.1.1.208 (Olympus).
And a change in lighting — from a stylized setup to ambient light, a long exposure and spot lights — helped.
The system is first calibrated radiometrically using both a diffuse white and dark target followed by a light setup based on the type of object to be recorded.
This current version of the approach is now able to 1) track multiple mobile sound sources 2) while employing a light hardware setup (only three microphones in a triangular array) and with a small computational resource requirement.
For AF studies, microscope excitation setup was adjusted to obtain a light fluence rate quite comparable to that used for microspectrofluorometric analysis (6.35 mJ cm−2 per second).
Such investments only make economic sense when imaging hundreds of thousands of specimens, and a more traditional system (e.g., a light box setup) will be more economical at smaller scales.
