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After the cells were settled, the solution was replaced with Tyrode's saline containing 2 µM fura-2/AM (Invitrogen) for 20 minutes and washed with normal Tyrode's.
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Meticulous explanatory panels now fill the elegant house that hosted the Wannsee Conference – when, on 20 January 1942, 15 senior Nazis settled the "final solution of the Jewish question in Europe".
Once the froth had settled and the solution had returned to room temperature, the substrate solution could be used in the assay for up to 6 h.
Glass jars with a hole cut in the lid work great and keep dust from settling into the solution.
It nonetheless settles the problem's solution because it applies to a decision problem if it applies to any accurate representation of the problem, such as Figure 1's representation of the problem.
Of his own health care law, Romney wrote in the op-ed: "Different states will experiment with and settle on the solutions that suit their residents best.
This process was conducted at 250 °C for 30 min then at 410 °C for 50 min followed by the addition of water (100 ml) to the solution after settling the temperature to an ambient value.
The correlations for GRF CO2 fracturing fluid rheological index, rheological coefficient, final proppant settling velocity in the solution and the critical settling velocity have been obtained with an average error of calculation less than 14%.
Parties will agree upon a final solution to settle the argument.
In HBSS, the hydrodynamic diameter of P25 aggregates ranged from 800 to 1,900 nm (30 min) and decreased to 770 nm (2 hr) as the larger aggregates settled from solution.
When the water reaches the surface, limestone settles out of the solution, forming the sparkling formations.
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CEO of Professional Science Editing for Scientists @ prosciediting.com