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Default settings were used for the three methods.
Moreover, the same high-performance software and hardware with the same settings were used for the computations.
It should be noted that the same parameter settings were used for both algorithms when possible in the simulations.
The following settings were used for both CT units: 1.0-mm reconstruction thickness, 1.0-mm space and 5.0-mm MIP slab thickness.
Default settings were used for all programs.
Default settings were used for all analyses except where specified.
Identical exposure settings were used for capturing images for each time-point.
Identical exposure settings were used for each stain and all photographs taken in one session.
Identical gain and contrast settings were used for all image acquisition.
These settings were used for further phylogenetic reconstruction and analysis of genetic diversity.
The resultant cell count for this case was ∼10 million and these settings were used for all the subsequent simulations on bifurcations.
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