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Web URLs, version information and parameter settings of the programs used in this study are summarized in Additional file 1: Table S3.
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The co-crystallized ligand was docked in its original protein structure using the default settings of the program.
The default settings of the program will perform well for the common use situations, but users who want more control over the search procedure can change the settings.
Docking was performed using the program HADDOCK [52], [53], with the default settings of the program.
The default settings of the program were used, including an admixture model.
For this analysis the default settings of the program were used to search the Pfam, Gene3D, and Superfamily databases.
Of the 192 clones sequenced, 148 (77.1%) contained microsatellites when searched using the default settings of the program Imperfect SSR Finder (Stieneke and Eujayl, 2007).
The 16S rRNA and cox1 sequences were aligned with default settings of the program Clustal X v1.83 [ 86]; ambiguously aligned sites in the 16S rRNA alignment were excluded.
For these analyses, the default settings of the program were used, but with polytomies randomly resolved according to a TSS-ERM with 1,000,000 permutations.
Compared to a similar set of maps constructed with a well-performing locus-ordering algorithm (RECORD) [ 26], the linkage groups built with JoinMap at the preset default settings of the program were inflated by 70±76%6%.
Both the command-line and the GUI version can save the current result and settings of the program into a file, which makes saving and modifying previous experiments simple.
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