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All primers (Additional file 7: Table S4) were designed from Repbase consensus sequences using default settings of Primer 3 and target regions represented in the crasiRNA pool.
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Four sets of primers viz.
We accepted the remainder of Primer 3's default settings.
Set the privacy settings of the event.
PCR primer pairs were designed using the Primer3 algorithm in the PrimerPro pipeline, with the following settings: primer length of 20 ± 2 nucleotides, GC content of 40 60%, and a PCR product size ranging from 100 to 300 bp.
Hodges finished his sandwich and opened a can of primer.
Then, apply a coat of primer.
Add a coat of primer.
Second, apply several coats of primer.
Apply the second coat of primer.
Optionally, apply a thin layer of primer.
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