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They showed that the performance of a delineation method depends on imaging parameters (i.e. reconstruction settings, image noise level, tumour characteristics, contrast) and you need to choose the suitable delineation method accordingly to get the optimum results.
After incubation, the plates were then loaded onto the Twister II Microplate Handler (PerkinElmer) and imaged on the Opera confocal microplate imaging reader (PerkinElmer) using the following settings; image size 0.430 × 0.345 mm, 20X water objective with a 405-nm excitation line and 420 490-nm band pass emission filter, laser power of 1,870 μW, and camera exposure times of 80 msec.
This finding suggests that for the considered geometry and settings, image non-uniformities are mainly imputable to the low-counting statistics.
Custom software (written as a Matlab™ graphical user interface) controls the laser power and wavelength, as well as all translational motion, image acquisition, gain and amplifier settings, image display, and online processing.
Camera settings, image display, and file saving are controlled with custom LabVIEW software.
Tumour boundaries from automatic delineation methods were affected by the choice of various settings, i.e. sphere size, TBR, reconstruction settings, image resolution and noise levels.
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To one side of the stage, Mr. Weil aims a video camera at small-scale models of the story's settings, images of which appear simultaneously at the back of the stage.
Taking into account that landscape change normally happens at a slow rate relative to the perceptive scale of humans, with fine grained changes taking place continuously in local settings, images of this kind present themselves as a way for the general public to become aware of changes which might otherwise be overlooked.
Secondly, we included a second "graphical shell" or "game world", which uses the same underlying game logic with different settings, images and characters.
Using a computerized system (Till Photonics) that controlled excitation wavelengths and camera settings, images of the analyzed areas (320 μm × 240 μm) were stored (frequency: 4 Hz, duration: 50 s) for each excitation wavelength.
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