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After ECMO support, daily arterial blood gas, ventilator settings, ECMO settings (gas flow, blood flow and FiO2) and ECMO complications (oxygenator failure, blood clots in oxygenator or circuit, bleeding, infection or others) were recorded until ICU discharge.
The mass spectrometer (MS) source parameters were optimized for each compound for the two most abundant multiple reaction monitoring (MRM) transitions under flow injection acquisition (FIA) mode using the following final settings: gas temperature 290 °C, gas flow 20 l/min, nebulizer 30 psi, sheath gas temperature 400 °C, sheath gas flow 12 l/min, capillary voltage −3000 V, nozzle voltage −2000 V.
At each sampling time we recorded data on ventilator settings, gas exchange, organ function and blood cell counts.
The plasma was used in continuous mode with following settings: gas flow 4 standard litres per minute (slm); supply voltage Usupl.
At baseline no significant differences were found in ventilator settings, gas exchange, respiratory mechanics, partitioned into its lung and chest wall components, as well as hemodynamics and EVLWI/ITBVI between normal IAP and IAH groups (Table 2).
Ventilatory settings, gas exchanges and haemodynamic parameters were recorded each time (from time 0 to time 8) in SP and PP: at the time of inclusion, before and immediately after each RM, before PP and one hour after turning the patient to SP. For this descriptive and analytical study, nonparametric tests were used.
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In clinical settings, gases are appreciated as primary variables in organ survival, with O2 as the critical gas parameter.
The electrospray ionization settings were gas temperature, 120 °C; gas flow, 15 L/min; nebuliser gas, 30 psi; sheath gas temperature, 375 °C; sheath gas flow, 12 L/min; capillary voltage, 3500 V; nozzle voltage, 300 V.
Mass spectrometer parameter settings were gas temperature (350°C), gas flow (10 L·minebulizerulizer (30 psi), sheath gas temperature (400°C), sheath gas flow (11 L·min−1), capillary voltage (3800 V) and nozzle voltage (500 V).
The ESI interface was used in positive ion mode with the following settings: sheath gas, 60 arbitrary units; auxiliary gas, 15 arbitrary units; sweep gas, 5 arbitrary units; capillary voltage, 4 kV; capillary temperature, 300 °C.
The DART interface was operated using the following settings: the gas heater was set to 200°C, the glow discharge needle was set at 3.5 kV.
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