Sentence examples for settings gap from inspiring English sources

Ninety-one mature miRNA sequences were used as custom sequences to search ESTs and perform target prediction using the program miRanda v1.0b [ 23] with the following parameter settings: Gap Open Penalty: −8.0; Gap Extend:−2.0; Score Threshold: 50.0; Energy Threshold: −20.0  kcal/mol; Scaling Parameter: 2.0.

Protein identity was determined using the "two sequence alignment" option of DNAMAN software (Lynnon BioSoft, Canada) with the following settings: gap open penalty 8 and gap extension penalty 2 and BLOSUM protein weight matrix.

Multiple sequence alignment of proteins was performed by ClustalX algorithm [ 49] with default parameter settings (gap opening penalty: 10, gap extension penalty: 0.2, delay divergent cutoff: 30%, protein weight matrix: Gonnet series) and this alignment was used to construct the phylogenetic tree using the neighbor-joining method in the MEGA package [ 50].

The pylogentetic tree was generated with the "Create Tree" function of CLC Main Workbench using the UPGMA algorithm with a bootstrap analysis of 100 replicates, based on an alignment of the HRP amino acid sequences using the "Create Alignment" function with the following settings: Gap open cost: 10.0, Gap extension cost: 1.0, End gap cost: as any other, Alignment: Very accurate (slow).

We used transAlign v.1.2 (Bininda-Emonds, 2005) to align protein-coding sequences (CYTB, COX1, and RAG2) by translating nucleotides to amino acids, aligning with clustalw v.1.83 with default settings (gap opening of 10, gap extension penalty of 0.2) (Thompson, Higgins & Gibson, 1994), and back-translating to nucleotides.

In the former settings, gaps in wireless networks would have to be assessed and the challenge addressed.

The protein-coding sequences were translated to amino acids and then aligned with CLUSTAL W under default settings for gap costs (gap opening penalty 10.00; gap extension penalty 0.20).

Sequences were unambiguously aligned in Clustal X [ 33] employing default settings for gap opening and extension costs.

Or an established system has not been designed to collect all of the important information to meet its intended purpose (scope gap) or is not covering the necessary populations or settings (coverage gap).

Through rigorous analysis and discussions in the fields of animal cloning and its relationship with tourism and tourists in potential future settings, a gap in the future market is presented.

In primary care settings, the gap of available providers is expected to continue to grow.