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The bioreactor settings for experiments were as follows: culture temperature was set at 37°C; maximum flow rate for O2 and CO2 were set at 10 ml/min; dissolved oxygen (DO) was set at 40% saturated air concentration; initial stirring speed was 60 rpm; initial pH were 7.1 and 7.3 for EX-CELL Vero SFM and OptiPro SFM respectively.
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To control for possible bleaching during multiple acquisitions, cells were imaged multiple times with the laser settings used for experiments.
Flow settings for the experiments were precalibrated using sets of whole blood from non-tumor bearing mice treated with 10,000, 1000, 100, 10, or 0 MDA-MB-231-GFP cells.
Because the MS3 settings for these experiments were different (neutral loss-dependent MS3 was used in this study in experiment 10_Alb_depl_NL only) we negated the MS3 results by removing single peptide-based protein identifications from both experiments, thus allowing a comparison based only on the addition of protease inhibitors.
The specific settings for the experiments are also provided.
Based on the analysis, we use random initial parameter settings for later experiments.
The compression settings for the experiments were the default settings of the reference software varying only quantisation settings and storage mode.
Then, we evaluate the impact of BG traffic on the performance of default NST-AODV with the routing metrics considered in Section 7. The radio chip settings for the experiments were those used in the previous section.
We used Beagle version 3.0.2 with default settings for all experiments presented here.
The microscopic settings for FLIP experiments were the same as for FRAP except that the bleaching event was performed with 200 ms laser duration and the exposure was 500 ms for Bem1-GFP and Bem1-GFP-CAAX probes.
The switches change settings very rapidly, effectively changing the detector settings for the experiment while the photons are in flight.
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