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with six pulses of 30 s each at the maximum setting (Library 1 and 2) or using a Covaris S2 (Covaris) for 8 minutes with the following settings: duty cycle: max, intensity: max, cycles/burst: max (Library 3, TBP, H3K4me3).
Three µg of genomic DNA in 100 µl TE-buffer was fragmented to a median size of 200 bp using the Covaris-S2 instrument (Covaris) with the following settings: duty cycle 10%, intensity 5, cycles per burst 200, and mode frequency sweeping for 180 s at 4°C.
with the following settings: Duty cycle 20, Intensity 5, cycles/burst 200, 45 seconds.
DNA fragmentation was targeted to 500-700bp model peak (AFA; Covaris settings: duty cycle 20%, intensity 5, cycle burst 200 for 30 s).
Ribosomal RNA was depleted using RiboMinus (Invitrogen), and the remaining RNA was sheared by ultrasonication on a Covaris S2 for 90 s with the following parameter settings: Duty Cycle=10, Cycles per Burst=200, Intensity=5.
DNA was sheared to a mean fragment size of 300 bp in Covaris 96 microTUBE plates (Covaris Inc., part no. 520078) using a Covaris E210 instrument with the settings: duty cycle 10, intensity 5, 200 cycles/burst, 60 sec.
Similar(53)
Goal-setting duties require the achievement of an outcome (e.g. a duty to ensure the safety and wellbeing of identified rights holders).
Chromatin was sheared 20 times for 45 s each (settings at duty cycle: 20%, intensity: 10, cycles/burst: 200) with 30 s of rest in between using a Covaris S2.
Approximately 5 µg C. elegans genomic DNA (quantified by NanoDrop) was fragmented to an average size of 200 bp using Covaris Adaptive Focused Acoustics technology with the settings: 20% Duty Cycle; Intensity 5; 200 Cycles per burst over the course of 3 minutes.
DNA was fragmented using a Covaris S2 sonicator (Woburn, MA, USA), with settings of 10% duty cycle, intensity 4, 200 cycles per burst and 120 s.
Genomic DNA was sonicated to 300-bp fragments with a Covaris S2 with the following settings: 10% duty cycle; 4 intensity; and 200 cycles/burst for 90 sec in 130 µl Tris-EDTA.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com