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In particular, the combination of cloud computing/network virtualization (including network functions virtualization) allow programmability that leads to unprecedented flexibility in rapidly creating, deploying, and managing novel services in virtualized settings as per the demands of users.
The DNA was extracted as per manufacturer's instructions, except for the following modifications: lysis buffer (AP1, RNase A and Reagent DX) and AP2 buffer volumes were increased 2.5× to allow for homogenisation of the tissue (50 mg) with the Geno/Grinder™ 2000 (settings as per Method C).
All RNAseq data were then pre-processed using the default settings of Just_Preprocess_My_Reads (http://justpreprocessmyreads.sourceforge.net) which conducted a mild quality control and trimming, pooled and assembled using Trinity-RNASeq using the default settings as per [ 33].
While the study shows that unintended pregnancy decreases with wealth, a result also observed in urban Kenya [ 30], it also reveals a higher prevalence in non-slums areas than in slum settings as per the bivariate results, a finding contrary to expectation, given the evidence that the slums of Nairobi are hubs of deprivation and risky health behaviors [ 20].
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These parameters were manually set as per the experimental settings done in [31] except that we used gray scale stereo images for training, i.e., P 0=1.
Collecting units consumed (e.g. capturing distance traveled instead of out-of-pocket fuel costs) facilitates portability of the results to alternate settings, as the monetary value per unit may vary depending on setting and region.
For measurements of lacuna density, individual 2 048 × 2 048-pixel frames were randomly captured throughout the tissue regions using a 10× (0.20 NA) objective and similar imaging settings as described above (0.44 μm per pixel).
Variability of alignment sites was quantified under default settings as recommended by the site's author (S. Kumar, pers. comm).
Arrays were processed as per manufacturer's instructions, scanned at medium PMT settings as recommended by the manufacturer, and analyzed using Bead Studio Software v. 2.3.41.
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