Sentence examples for settings a gap from inspiring English sources

Through rigorous analysis and discussions in the fields of animal cloning and its relationship with tourism and tourists in potential future settings, a gap in the future market is presented.

Evidence about task division between CCWs and PCGs is limited in resource-poor settings, a knowledge gap that should be addressed.

The general lack of clarity and diversity in models, roles and care settings have resulted in a gap in knowledge about what makes for effective interventions for the development of the support workforce.

ABC protein sequence similarities and identities were calculated using MatGAT 2.03 [ 127] using default settings (BLOSUM50 matrix, gap opening and extending gap penalty set to 12 and 2, respectively).

The following search settings were employed: gap opening penalty = 11; gap extension penalty = 1.

Multiple sequence alignment of proteins was performed by ClustalX algorithm [ 49] with default parameter settings (gap opening penalty: 10, gap extension penalty: 0.2, delay divergent cutoff: 30%, protein weight matrix: Gonnet series) and this alignment was used to construct the phylogenetic tree using the neighbor-joining method in the MEGA package [ 50].

We used transAlign v.1.2 (Bininda-Emonds, 2005) to align protein-coding sequences (CYTB, COX1, and RAG2) by translating nucleotides to amino acids, aligning with clustalw v.1.83 with default settings (gap opening of 10, gap extension penalty of 0.2) (Thompson, Higgins & Gibson, 1994), and back-translating to nucleotides.

The indicated L2 residues were aligned with the MacVector software ClustalW algorithm using the BLOSUM series matrix with default settings for open gap penalty, extended gap penalty, delay divergent percentage, and protein gap parameters.

For Additional Figure 1, default settings were used except for using a PAM matrix, a gap opening penalty of 2 and a gap extension penalty of 0.05.

Ninety-one mature miRNA sequences were used as custom sequences to search ESTs and perform target prediction using the program miRanda v1.0b [ 23] with the following parameter settings: Gap Open Penalty: −8.0; Gap Extend:−2.0; Score Threshold: 50.0; Energy Threshold: −20.0  kcal/mol; Scaling Parameter: 2.0.

The resultant multiple alignments were trimmed with TrimAl v1.2 [ 22] with five different settings: (1) no gap allowed, (2) no gap allowed and the similarity threshold 'st' of 0.00005, (3) no gap allowed and st = 0.0001, (4) no gap allowed and st = 0.0005, and (5) no gap allowed and st = 0.001.