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Finally, based on the analysis of problems resulted from setting threshold of a decision function in the VCA method and proposed an improved decision function on the basis of sorted value of vanishing component polynomial, the experiment showed that such improved decision function was more operational.
Having a representative core gene set of depression is essential to the final gene selection, as the numbers of weight matrices that satisfied the selection criteria were correlated with setting threshold of φ (proportion of core genes).
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We set threshold of route recalculation θ V to be 0 [m/s].
This avoids the problem of not easily set threshold of classification decision function for the VCA method and enhances feasibility in real application.
In ImageJ this corresponded to a set threshold of baseline-75 for the cortical region and baseline-24000 for the striatal region.
Consequently, we tested across these with a set threshold of p < 0.025 for each tail.
*Boldface type indicates codes that achieved the a priori set threshold of a positive predictive value ≥ 50%.
We considered, for further analyses, a miRNA as expressed only when both biological replicates met the set threshold of 10 TP5M.
Standard curves were created for all genes measured and a set threshold of 0.15 was used for all calculations of relative gene expression.
CT values were defined as the number of cycles required for normalized fluorescence to reach a manually set threshold of 20% total fluorescence.
Fluorescence signals were analysed using an automatic setting of the threshold line in the 7500 software (v.2.0.4) and a manually set threshold of 0.01 in the softwares of the Stratagene and Rotor-Gene real-time PCR instruments.
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