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However, the setting specificity of these two studies limits the generalisability of their findings.
At the final or first level of goal setting, specificity matters.
In a clinical setting, specificity, sensitivity and convenience are necessary for treatment planning.
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Melting curve analysis was performed to evaluate primer set specificity.
Top probe sets were selected based on probe set specificity, strength of association to the Comt locus, biological function, and expression pattern for each relevant brain region in the AIBS (www.brain-map.org).
Although the sensitivity of syndromic surveillance was high when we set specificity to 0.90, this specificity resulted in a false alarm (false-positive detection) ≈1 every 10 days.
We set specificity of trypsin digestion and allowed 2 missed cleavage sites and we set the mass tolerances in MS and MS/MS to 2 ppm and 0.5 Da, respectively.
HE4 showed a considerably higher sensitivity compared with CA125 for detecting EC, considering all stages and all set specificities.
The combination of CA125 and HE4 led to a further improvement in sensitivity, even if limited, compared with HE4 alone, considering all set specificities and all EC stages.
Setting the specificity level at 95% resulted in a decrease in sensitivity except for A. baumannii (it reached nearly 100%); it fell below 50% for three pathogens: around 40% for Streptococcus pneumoniae and Enterococcus faecalis and 25% for Enterobacter cloacae.
The (age-specific) threshold values were also computed based on two different criteria: (a) by setting the specificity at 0.7, and (b) by the Youden Index (YI).
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