During division, the genome sets the polymerase rate and directionality of the newly synthesized copy.
While in the face of the 2009 pandemic it makes sense that N eτ for both the HA and NA antigens increased, it is unclear (i) why N eτ decreased for some segments and (ii) why a decoupling is inferred within the polymerase genes, setting PB2, which has a role in host restriction (e.g., [ 17]), apart.
The predictions of the best models are shown in Figure 2. Using on average 80% of the 34 datapoints in the polymerase set, the model predicted expression from the remaining 20% of sequences with a correlation coefficient of 0.69 (Figure 2A).
A metaphor for this knowledge set is the polymerase chain reaction (PCR).
Genomic DNA (gDNA) was extracted from livers of the eight individuals comprising the P generations of each cross using standard protocols and was PCR amplified (20 μL reaction volume) for each primer set using AmpliTaq Gold polymerase (Invitrogen).
The crystal structures of the HCV NS3·4A protease and the NS5B RNA polymerase set a strong foundation for rational structure-based drug design, which in turn was critical for optimizing protease potency and allosteric binding.
One set of polymerase contacts, between the "thumb" domain of one polymerase and the back of the "palm" domain of another, has been previously defined.
A set of polymerase chain reaction (PCR) primers that spanned the (CTG n repeat region in exon 1 was used to produce DNA fragments.
Whereas sequence studies of microsatellites rely on complete or partial genome sequences, typical microsatellite studies of genetic diversity instead use data sets of polymerase chain reaction (PCR) fragment lengths measured for microsatellite loci in a collection of individuals.
Apolipoprotein E (APOE) genotype was determined in approximately half of the sample set using polymerase chain reaction amplification of leukocyte deoxyribonucleic acid followed by HhaI digestion and product characterization (Hixson & Vernier, 1990) and by TaqMan in the remaining half, relying on several single nucleotide polymorphisms around the APOE gene (Koch et al., 2002).
