Figure 3 illustrates representative results from a set of lymph nodes from a single patient.
AP4 is statistically significant on the set of lymph node negative tumors that are < 2 cm in diameter).
Seven biomarker candidates, CEA, CEACAM1-S/L, CEACAM6, CEACAM7-1/2, MMP7, MMP7 and CK20, were compared in a test-set of lymph nodes from 51 CRC patients (Dukes' A D) and 10 controls.
With mice placed in prone positions, both right and left sets of lymph nodes were surgically removed in the following order: the left popliteal, the left lumbar, the right lumbar, and the right popliteal.
We, therefore, investigated a number of biomarkers, that are expressed in colon adenocarcinoma cells and compared their expression levels in a test-set of lymph nodes from CRC patients of different Dukes' stages and controls as well as in primary tumours, in epithelial cells of normal and inflamed colon and in different types of immune cells.
The potential to use such methodologies, in the setting of axillary lymph node negative breast cancer, to inform the decisions regarding chemotherapy is currently being tested in prospective randomized trials [ 1, 17- 21].
For patients with ER-positive, HER2-negative disease, the use of molecular diagnostics was felt to be unnecessary in low-risk patients such as those with a tumour size of ≤1 cm in the setting of negative lymph nodes, since chemotherapy would be unlikely to be given anyway.
Like the TRANSBIG data set, the Wang data set is comprised of lymph node negative patients.
Outliers for kappa lambda light chain ratio, CD10 CD19 coexpression, CD5 CD19 coexpression, CD4 CD8 ratios and CD7 loss were summarised for histological pattern, concomitant diseases and follow-up course.We generated the largest data set of benign lymph node immunophenotypes by an order of magnitude.
The set of inguinal lymph nodes from each mouse was pooled and used in each assay.
