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Here, equal amounts of DNA from patients and controls are mixed to form two sets of pools.
Briefly, in DNA pooling equimolar amounts of DNA are taken from each individual mixed to form two sets of pools, cases and controls.
However, for the log-normally distributed data, a minimum of four sets of pools are required because the increased number of pools caused their means to be more normally distributed [ 27].
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Four sets of pooled samples were prepared for hybridization to separate Affymetrix rat genome 230 2.0 arrays (i.e. four separate sets of samples were analysed for each condition, prepared from a total of 12 myocyte preparations).
The software program "Deconvolute This" was used to create sets of pooled peptides, such that each peptide from the overlapping 15-mers (derived from the three envs, gag, pol, and nef antigens contained in the vaccine) is present in five different pools.
Each of these two subsets included two sets of pooled RNA samples from either needle tissue or from wood tissue.
Three sets of pooled samples were analyzed for each genotype [wild type, heterozygous, knockout ] and age (7 and 10 months).
All hepatopancreatic BBMV data in this investigation were obtained using at least 3 sets of pooled frozen BBMV preparations.
The Baz1b results were reproduced in two independent sets of pooled samples, and by testing the individual samples separately.
To permit an accurate determination of the values in controls, we therefore created four separate sets of pooled samples, each composed of CSF taken from 3 4 Gfap +/+ mice.
Our analyses provide two sets of pooled estimates for both ICU readmission and hospital mortality that offer a range of potential benchmarks.
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