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Three independent sets of ChIPs with c-Myc antibody versus input DNA were hybridized to the custom array.
Data from outlier chips were discarded and hybridisations were repeated with the same or fresh RNA samples from the same batch of mice until complete sets of chips for each condition were obtained.
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Three sets of ChIP-chip data were used in this study.
Not only are the sets of ChIPed fragments different between the T3 leg and haltere, the associated DNA sequence motifs are also distinct.
Our pipeline uses GADEM [20], which is a good compromise between fully probabilistic and enumerative approaches, can process large sets of ChIP-Seq regions, handles both dimer and monomer motifs, automatically identifies multiple motifs, and automatically adjusts motif widths.
Because PICS is based on a formal statistical model that requires an EM algorithm for estimating the unknown parameters, we have designed the R package PICS to be computationally efficient enough to process large sets of ChIP-Seq reads.
PCR was performed on unamplified DNA samples of several sets of ChIP experiments.
Second, there is a limitation in showing multiple sets of ChIP-seq data for different projects separately.
A newly developed method jMOSAiCS [ 8] implements a joint model for multiple sets of ChIP-seq data.
Both sets of chip samples were screened over a gyratory screen with a woven-wire mesh bottom screen with 3 mm openings to remove the fine particles.
Only nine Zfp335-bound regions showed decreases in ChIP-seq peak intensities in blt/ blt thymocytes that were robust enough to be detected across both sets of ChIP-C and ChIP-N replicates.
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