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Two sets of capture probes were designed.
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From the sheer quantity of captured equipment, Knights noted "ISIS has probably three sets of captured M16 rifles and body armor for every one of its fighters".
From the sheer quantity of captured equipment, Knights notes "ISIS has probably three sets of captured M16 rifles and body armour for every one of its fighters".
A consignment of 11,500 sleeveless leather jerkins for protection against the cold and barbed wire were distributed, as were 350 sets of captured Italian wire cutters.
As many epitopes have a very large capacity, and possibly cover up to a few hundred peptides from many different proteins, it is more probable that the sets of captured proteins overlap.
After enrichment with each individual kit, captured fragments were purified, pooled in two groups (each containing the two sets of captured regions from each of 10 different samples), and sequenced on two lanes of an Illumina HiSeq 2000 System with the use of 96-bp paired-end reads.
The basic data required are a set of capture histories of individually identified animals.
Therefore, our goal was to control as many environmental factors as possible to use the most equivalent set of capture conditions across the various sensors while maintaining a well-calibrated image in each spectral band.
The first set of capture probes consisted of six high specific 50-mer probes (P1 P6) targeting six distinct regions of the M. acetivorans C2A mcrA gene (Fig. 2, Supplementary Table S1).
According to the alignments, Salmonella and non-pathogenic E. coli strains could be clearly typed based on the probes included on the microarray although their genome sequences did not cover the full set of capture probes designed for these groups.
Finally, we note that the development of a single set of capture probes for a pathogen of interest will enable generation of pathogen WGS directly from vector and host samples (whether human, animal reservoir, or both), enabling study of host specificity and potential transmission bottlenecks.
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