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Yet, the different sets of alignment parameters investigated here do not have similar tolerance for highly divergent reads.
In practice we used two sets of alignment filters and kept alignments that passed one or both sets.
Stitch: automated and manually edited assemblies Tcas5.1 is the output of Stitch (version 1.4.4) run for five iterations with two sets of alignment filters.
Dating analyses were performed on three sets of alignment data; RAG-1 nuclear data only (nuc), nuclear and mtDNA data combined (comb), and nuc and mtDNA combined with 3rd positions removed from the mtDNA dataset (comb reduced).
It performs sequence alignments using the program MAFFT [ 84] using different sets of alignment parameters to create three different alignments for each gene family and culls [ 85] alignment ambiguous regions.
Comparison of mean and range (95% posterior density distribution) of divergence time estimates for selected outgroup and Crenadactylus nodes based on Bayesian dating analyses (BEAST) of three different sets of alignment data.
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In the case of submitting sequences, they are first combined together, aligned using MUSCLE (Edgar, 2004) and then split into two separate sets of alignments.
Cumulative Relative Entropy (CRE) calculated for these two sets of alignments enabled us to selectively identify conserved residues of not only CaMdr1p but for the entire DHA1 family.
While these two measures alone are not powerful enough to produce high quality results, they can be used to expand the solution sets of alignment-based methods and thus increase their coverage.
Two sets of alignments were considered for the phylogenetic analyses.
We applied the same processing and analysis steps to both sets of alignments.
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