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In practice, user can submit a customized reference list when the default background is inappropriate (e.g., if it is desired to use expressed probe sets as a background, while only a portion of probe sets are expressed in a typical expression profiling experiment).
GO enrichment analysis, using these 15 target genes against the HG-U133 Plus 2.0 array gene sets as a background in DAVID, revealed angiogenesis as a broad enrichment for the nine SP3 targets, and mesenchymal cell proliferation for the six NFκB1 targets.
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Production of cytokines by unstimulated cells set as a background was subtracted from all measurements.
We also used the whole RefSeq gene set as a background control.
In UPS 2.0, Unigene data are updated, and UPS 2.0 can take the user-uploaded sequence set as a background reference.
To see whether a sub-cellular location was overrepresented, the proteins encoded by the longest transcripts of maize AGPv2 5b filtered datasets [ 16] were set as a background for the analysis.
For all simulation scenarios, the level of exposure through ingestion of contaminated food was set as a background daily exposure of 10 ng/kg body weight/day (unless specified otherwise).
Comparative GO analysis of 1st and 4th quartile miRNA target gene sets was carried out using Gorilla web tool (http://cbl-gorilla.cs.technion.ac.il/) [ 47], by setting 4th quartile gene set as the list of interest and 1st quartile gene set as a background set and vice versa.
GO term over-representation testing of the TNR-encoded homo-AA proteins was initially done using the variant-encoded homo-AA protein set as a background model; this test identifies whether the TNR-encoded set is significantly different from the variant-encoded set.
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Previous GO over-representation analyses of TNR se- quences have used whole organism gene sets as a statistical background [ 12].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com