In a previous work [ 12], we showed that yeast strains deficient in ergosterol synthesis (Δerg6 strains) are particularly sensitive to daunorubicin, overcoming one of the main setbacks to the use of yeast in pharmacological studies, which is their resistance to many anti-tumour drugs [ 13, 14].
But it is evident that the establishment of pure Eucalyptus plantations on a large scale creates a risk of possible serious attacks that could result in a dangerous setback to the economic use of these quick-yielding species.
One approach rapidly gaining support to overcome this setback is the use of in silico (computer-based methods) evaluation of the drug metabolism and pharmacokinetic (DMPK) profiles of these compounds early in the preclinical drug discovery/development pipeline [10, 11, 12, 13, 14].
The decision by Lord Justice Richards and Mr Justice Mitting in the high court will have far-reaching consequences and is a severe setback for the use of material obtained from leaks or whistleblowers.
These setbacks necessitated the use of an improved extraction method involving lower solvent consumption, shorter irradiation time, higher yield, higher selectivity, and environmental friendly.
In view of these considerable setbacks with the use of SARM KO mice, we strategically planned and developed a novel in vivo adoptive transfer mouse model system to study the effect of SARM knockdown during an experimental influenza infection.
The accident in Fukushima Daiichi Nuclear Power Plant caused setback to the credibility and reliability of nuclear power.
However, the major setback that usually foils the use of this carbon source is when treating low COD/N wastewaters, e.g. supernatants from sludge digesters and stabilization ponds as well as pretreated industrial wastewaters by anaerobic fermentation, in which external carbon source is frequently added to spur the denitrification activities.
The threat of Ebola in West Africa is still a major setback to the socioeconomic development.
But if the policy change could bring in more money, a legal setback in one of the cases involving the use of trademarks to activate ads could be a serious blow to Google's business.
