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The alignment of the experimental 50 nt-reads was set to tolerate a maximum of six color-space mismatches.
The clean reads were realigned to the BN genome with SOAP2 set to tolerate gaps of up to 10 bp.
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"Our mouths are set up to tolerate a large amount of insults," Joseph Califano, a head-and-neck surgeon at Johns Hopkins, says.
In non-sedated patients, intensity was initially set to the maximum tolerated level and was increased by 10%% (or less in case of discomfort) every 3 min throughout the session.
For example, if the number of failure to tolerate is set to 1, the storage management system will copy data generated by that server in a physically separated storage [26]. 2.
For annotation of the molecular formula of precursor ions, the maximum tolerated mass deviation was set to ±3 ppm.
The tolerated polymorphism rate was set to 5%, and thus sequences more divergent than 95% were separated and those more similar than 95% were grouped together.
The non-inferiority margin was set at 5% to tolerate an increase in local recurrence to 12% in the experimental arm.
STAR was run with the following parameters: minimum/maximum intron size set to 30 and 500,000, respectively, noncanonical, unannotated junctions were removed, maximum tolerated mismatches was set to 10, and the outSAMstrandField intronMotif option was enabled.
As even the strictest parameter set (i.e. the set that tolerates least errors in the data) together with all others flags this assignment as critical, its overall criticality is set to very critical.
Further, parameter setting is advised to tolerate stuck-at-close defects.
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CEO of Professional Science Editing for Scientists @ prosciediting.com